In 2010, ONT combined two technologies addressing each of the main outstanding problems. The first was an engineered nanopore developed in collaboration with Bayley’s lab that could discriminate between individual DNA bases, solving the resolution issue. The second was a trick to slow the DNA down to detectable speeds, using the familiar DNA polymerase enzyme. Mark Akeson’s lab at UC Santa Cruz had identified a specific polymerase from the bacterial virus ɸ29 that replicated DNA at an ideal speed for detection via nanopore. Template DNA strands were replicated just before entering the nanopore, passing through slowly enough for individual bases’ effect on the electrical current to be detectable and allowing the DNA sequence to be read one base at a time.
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The bubonic plague, which swept across Europe between 1347 and 1353, is estimated to have killed up to one half of the continent’s population. The sudden loss of life led to the abandonment of farms, villages and fields, creating what researchers describe as a massive historical ‘rewilding’ event.，这一点在WPS下载最新地址中也有详细论述
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